Annals of Neurosciences ANS Annals of Neurosciences 0972-7531 0976-3260 Indian Academy of Neurosciences 220213 10.5214/ans.0972.7531.220213 Molecular Shots Biomolecular Imaging Immunodetection of oligoclonal bands in serum and CSF of Multiple Sclerosis patients 1 2015 22 1 54 54 Copyright © 2015, The National Academy of Sciences 2015 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Immunodetection of oligoclonal bands in serum and CSF of Multiple Sclerosis patients. Pattern 1: OCBs in CSF only (positive): Oligoclonal bands present in CSF only. Intrathecal IgG synthesis as seen in MS; Pattern 2: No OCBs seen (negative, policlonal): No oligoclonal bands in CSF or Serum. No intrathecal Ig synthesis; Pattern 3: Identical OCBs in both (mirror): Bands in serum mirror those in CSF. This suggests systemic Ig synthesis; Pattern 4: Identical OCBs in both with extra in CSF (more than): Identical bands in both serum and CSF with extra bands in CSF. Image demonstrates both intrathecal and systemic Ig synthesis. This is identical as it is seen in MS.

Indirect immunofluorescence in cells transfected by aquaporin4 (EUROIMMUN Aquaporin-4 IIFT). Panel A: Anti-AQP4 antibodies observed in the serum of NMO patients (positive sample); Panel B: Absence of anti-AQP4 antibodies in serum sample (negative sample)

By María Simó-Castelló1, Carmen Alcalá1, Deepali Mathur2, Gerardo López-Rodas3, María Burgal Marti4, Bonaventura Casanova1

1 Hospital Universitari i Politècnic La Fe, València, Spain

2 Department of Functional Biology, University of Valencia, Valencia, Spain

3 Department of Biochemistry and Molecular Biology, University of Valencia, and INCLIVA Biomedical Research Institute, Valencia, Spain

4 Multiple Sclerosis Laboratory, Department of Biomedicine, Prince Felipe Research Center, Valencia, Spain